Calendar
Lecture: "Latest techniques for manipulating single DNA molecules"
Monday, 29 March, 3:05 PM » 4:05 PM
Site event
Prof. Akira Mizuno from Toyohashi University of Technology
This visit to Scotland has been made possible by the Leverhume Foundataion, and the lecture on Monday will be made available in all of the SUPA video conference lecture rooms.
Aberdeen, Old Aberdeen RG17
Heriot-Watt, room EM1.27
Dundee, Ewing Building video conference room
UWS Henry F318
Strathclyde JA 8.13
Edinburgh JCMB 6224
Glasgow Kelvin Building room 255
St Andrews Physics room 307
This has been distributed to staff and research students in Physics departments in SUPA, but the lecture is open. Please feel free to pass on the invitation to colleagues in related subjects such as Engineering, Chemistry or Biology.
Summary Abstract:
Latest techniques for manipulating single DNA molecules
Akira Mizuno
Dept. Ecological Engineering, Toyohashi University of Technology,
Hibarigaoka 1-1, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan
Single-molecule imaging and single-molecule manipulation techniques have been investigated to analyze various biological phenomena and information. In particular, direct observation of fluorescent-labeled single DNA molecules can be used for mapping, or for investigations on interactions with proteins and dynamics of biomacromolecules. For these purposes, single-molecule DNA manipulation is also a crucial technique. Because the form of DNA molecules always changes in solution by Brownian motion, a “physical form control” technique is required to analyze individual DNA molecules. For example, fluid flow, moving meniscus, optical trapping, or electric force can be used. At the same time, miniaturized chemical reaction systems such as micro total analysis systems (micro-TAS) have been actively studied in recent years. Fluid control is one of the most important aspects of microchannel networks, and various types of micropumps that can be integrated on a chip have been proposed. In addition, W/O emulsion system has attracted attention as a tool for miniaturized chemical reaction. In this system, each droplet is served as a micro-reactor containing different materials. It has been applied in combinatorial chemistries, for example, direct evolution of protein from gene library. In addition, when small amount of substrates such as single molecule are encapsulated in the droplets, effective concentration of the substrates can be increased, resulting in increased efficiency of chemical reactions. For example, single molecule polymerase chain reaction (PCR) was achieved using W/O emulsion, whereas conventional PCR did not succeed.
Some of those techniques developed in our laboratory will be presented in this lecture.
pdf of abstract and workshop: http://connect.supa.ac.uk/file.php/2/Current_resources/mizuno-abstract-2010.pdf
This visit to Scotland has been made possible by the Leverhume Foundataion, and the lecture on Monday will be made available in all of the SUPA video conference lecture rooms.
Aberdeen, Old Aberdeen RG17
Heriot-Watt, room EM1.27
Dundee, Ewing Building video conference room
UWS Henry F318
Strathclyde JA 8.13
Edinburgh JCMB 6224
Glasgow Kelvin Building room 255
St Andrews Physics room 307
This has been distributed to staff and research students in Physics departments in SUPA, but the lecture is open. Please feel free to pass on the invitation to colleagues in related subjects such as Engineering, Chemistry or Biology.
Summary Abstract:
Latest techniques for manipulating single DNA molecules
Akira Mizuno
Dept. Ecological Engineering, Toyohashi University of Technology,
Hibarigaoka 1-1, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan
Single-molecule imaging and single-molecule manipulation techniques have been investigated to analyze various biological phenomena and information. In particular, direct observation of fluorescent-labeled single DNA molecules can be used for mapping, or for investigations on interactions with proteins and dynamics of biomacromolecules. For these purposes, single-molecule DNA manipulation is also a crucial technique. Because the form of DNA molecules always changes in solution by Brownian motion, a “physical form control” technique is required to analyze individual DNA molecules. For example, fluid flow, moving meniscus, optical trapping, or electric force can be used. At the same time, miniaturized chemical reaction systems such as micro total analysis systems (micro-TAS) have been actively studied in recent years. Fluid control is one of the most important aspects of microchannel networks, and various types of micropumps that can be integrated on a chip have been proposed. In addition, W/O emulsion system has attracted attention as a tool for miniaturized chemical reaction. In this system, each droplet is served as a micro-reactor containing different materials. It has been applied in combinatorial chemistries, for example, direct evolution of protein from gene library. In addition, when small amount of substrates such as single molecule are encapsulated in the droplets, effective concentration of the substrates can be increased, resulting in increased efficiency of chemical reactions. For example, single molecule polymerase chain reaction (PCR) was achieved using W/O emulsion, whereas conventional PCR did not succeed.
Some of those techniques developed in our laboratory will be presented in this lecture.
pdf of abstract and workshop: http://connect.supa.ac.uk/file.php/2/Current_resources/mizuno-abstract-2010.pdf
